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. 2012 Feb 1;287(13):10001–10012. doi: 10.1074/jbc.M111.322552

FIGURE 6.

FIGURE 6.

M-type channel activity stimulated by LPA. A and B, exemplar traces of M-channel recordings showing effects of sequential increases in LPA concentrations applied to the inner leaflet of excised inside-out patches from CHO cells stably expressing M-type Kv7.2/7.3 channels (holding potential of 0 mV). C, closed state; O1–O5, open state currents for one to five channels. C, mean ± S.E. for Po of M-channels plotted against LPA concentration. – – –, diC8PI(4,5)P2 (from Fig. 2); ♦, LPA; ♢, 100 μm diC8PI(4,5)P2 (reference point for LPA). The data points were fitted to a two-component Hill equation (see “Experimental Procedures”) with the parameters listed in Table 2.