Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
NOX4 is the major isoform in 3T3-L1 adipocytes. Differentiated 3T3-L1 adipocytes were cultured for 7 days in low or high glucose media with or without 250 μm of palmitate or DHA. Total RNA was isolated and analyzed by multiplex real time RT-PCR using various Nox-specific primers and probes as indicated (A and C) and normalized to Gapdh. NOX4 protein levels of cells were measured by immunoblot using an anti-NOX4 antibody (B). *, p < 0.001 versus Nox1–3 and -5 and Duox1/2.
