FIGURE 4.

Loss of Nrf2 elevates intracellular ROS levels. Nrf2^+/+ and Nrf2^−/− iDCs were untreated (basal) or treated with LPS (1 μg/ml) or H₂O₂ (1 mm) for 10 min at 37 °C. Cells were then incubated with the ROS indicator dihydroethidium before analysis by flow cytometry. Data points represent mean fluorescence intensity of six separate measurements from two independent experiments. The horizontal line represents the mean. Statistical significance was tested by unpaired Student's t test (*, p < 0.05).