FIGURE 5.

Loss of Nrf2 perturbs NF-κB signaling in iDCs. Nrf2^+/+ and Nrf2^−/− iDCs were treated with LPS (1 μg/ml) for the indicated times, and whole cell lysates were subjected to SDS-PAGE. A and B, the levels of phosphorylation of p65 (p-p65) (A) and of IκBα levels (B) were assessed by Western blotting. Data are representative of three independent experiments. C, NF-κB activity in the nuclear lysates of Nrf2^+/+ and Nrf2^−/− iDCs was measured and presented as dot plots with each dot representing mean relative luminescence units (RLU) (from triplicates). Data are from six independent experiments. The horizontal lines indicate means for each group.