FIGURE 6.

Effects of Nrf2 loss on MAPK signaling in DCs. Nrf2^+/+ and Nrf2^−/− iDCs were treated with LPS (1 μg/ml) for the indicated time points, and whole cell lysates were subjected to SDS-PAGE. A, panel i, phosphorylation of ERK1/2 (p-ERK1/2) assessed by Western blotting. B, phosphorylation of p38 MAPK (p-p38) assessed by Western blotting. Data are representative of three independent experiments. A, panel ii, Nrf2^+/+ and Nrf2^−/− iDCs were untreated or treated with 50 μm PD98059 (MEK1 inhibitor/ERK inhibitor). Co-stimulatory receptor expression was determined by flow cytometry. Data are representative of three independent experiments.