FIGURE 7.

Histone deacetylase function is required for co-stimulatory receptor expression in Nrf2^−/− iDCs. A, Nrf2^−/− iDCs were treated with the HDAC inhibitor, valproic acid (100 μg/ml) (+VPA), for 72 h and labeled with antibodies against MHC II and CD86. The percentage of DCs expressing high levels of co-stimulatory receptors is indicated above the marker. Representative histograms are presented with average percentage ± S.D. Data were derived from four independent experiments. B, Nrf2^−/− iDCs were untreated or treated with VPA for 24 h, supernatants were collected, and levels of TNF-α were measured. Data are presented as average pg/ml ± S.D. and derived from three independent experiments. C, HDAC activity was measured in nuclear lysates from Nrf2^+/+ and Nrf2^−/− iDCs. Data are presented as average absorbance at 405 nm ± S.D. and derived from three independent experiments. OD, optical density.