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. 2012 Feb 6;287(13):9845–9854. doi: 10.1074/jbc.M111.250357

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Cell fate decisions after p21 up-regulation do not depend on differences in p21 expression or ROS levels. A, immunoblot analysis of HT1080p21–9, EJp21, and PC3p21 uninduced (−) and induced to express p21 for 5 days (+). B, top panel: intracellular ROS levels as measured by DCF staining of HT1080p21–9, EJp21, and PC3p21 cells induced for 5 days. Bars represent fold changes in mean fluorescence intensity from control to induced cells. Bottom panel, percentage of events in the sub-G₁ phase, as measured by PI staining of the same cells.