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. 2012 Jan 27;287(13):10265–10275. doi: 10.1074/jbc.M111.325175

FIGURE 3.

FIGURE 3.

Activation of ERK is required for mGluR7 reduction of NMDAR currents. A, plots of normalized (Norm.) peak NMDAR currents in dissociated PFC pyramidal neurons showing the effect of l-AP4 (200 μm) in the absence or presence of PKA activator cpt-cAMP (100 μm) or PKA inhibitor PKI14–22 (PKI, 0.1 μm, myristoylated). ctl, control. B, plots of normalized peak NMDAR currents showing the effect of l-AP4 (200 μm) in the absence or presence of ERK kinase inhibitors U0126 (20 μm) or PD98059 (50 μm). C, cumulative data (mean ± S.E.) showing the percentage of reduction (% reduc.) of NMDAR currents (curr.) by l-AP4 in the presence of cpt-cAMP, PKI14–22, U0126, PD98059, wortmannin (1 μm), or LY294002 (100 μm). *, p < 0.001, ANOVA. D, top, Western blotting of phospho-ERK (p-ERK) and total ERK in cultured PFC neurons showing the effect of l-AP4 in the absence or presence of CPPG or PD98059. Bottom, quantitative analysis showing the -fold increase of phospho-ERK by l-AP4 under different conditions. *, p < 0.001, ANOVA.