FIGURE 4.

The human PAI-2 transactivator AP-1 site binds multiple Jun and Fos family proteins. Supershift assays were performed with nuclear extracts prepared from U937 cells (A) and HeLa cells (B) cultured in the presence (+) or absence (−) of PMA for 4 h and using the ³²P-labeled double stranded −4952/−4932 oligonucleotide as a probe. Nuclear extracts were preincubated with 4 μg of specific antibody at 4 °C for 2 h. Images have been inverted for clarity. C, table summarizing the findings from three sets of independent supershift experiments.