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Transient expression of FosB, c-Jun, and c-Fos induces low level expression of the endogenous PAI-2 gene. HeLa cells were transiently transfected with the indicated expression plasmids in the presence of PMA. RNA was isolated, and PAI-2 mRNA was detected by quantitative PCR. PAI-2 Ct values were normalized to that of β-actin.
