FIGURE 8.

Mapping the residues within transmembrane helix 4 that are required for PAR4 homodimers. HEK293 cells were transfected with HA-PAR4-Luc (0.03 μg) and V5-PAR4-GFP (0–0.24 μg) (A), V5-PAR4–2A-GFP (0–0.24 μg) (B), or V5-PAR4–4A-GFP (0–0.24 μg) (C). PAR4–2A is PAR4-L209A/L213A, and PAR4–4A is PAR4-L192A/L194A/M198A/L202A. Forty-eight hours post-transfection, the cells were analyzed for GFP expression, luciferase expression, and BRET. The data are from three or four independent experiments in which all points were analyzed by global fit to a hyperbolic or linear curve.