TABLE 2.

BRET_max values for PAR4 point mutants

PAR4 variants were tagged with GFP at the C terminus and examined in BRET assays with PAR4-wt-Luc. The data were fit to a hyperbolic curve, and each PAR4 variant was compared with PAR4-wt.

PAR4 variant	BRETmax	95% confidence interval
PAR4-wt	0.46	0.23–0.70
PAR4–2A	0.07	0.02–0.12
PAR4–4A	Linear
PAR4-L192A	0.55	0.44-.066
PAR4-L194A	0.39	0.25–0.53
PAR4-M198A	0.31	0.23–0.40
PAR4-L202A	0.31	0.05–0.57