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. 2012 Feb 7;287(13):10435–10443. doi: 10.1074/jbc.M111.299669

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Altered epidermal differentiation of Jup mutant mice (16 days old). A, markedly thickened Jup mutant (Mut) suprabasal layer (panel e) compared with the control (Ctl; panel b) as revealed by involucrin staining. Scale bars = 30 μm. B, significantly expanded basal layer in Jup mutant epidermis (panel e) compared with the control (panel b) as shown by keratin 5 staining. Scale bars = 30 μm. WGA, wheat germ agglutinin. C, Western blot analyses of involucrin, keratin 5, and keratin 14. GAPDH served as a total protein loading control.