TABLE 2.

Specificity of recombinant CNL for glycans determined by glycan microarray analysis

Specificity was defined using concentration-dependent binding of the lectin to glycan microarrays with concentrations of 10, 1, and 0.1 μg/ml (29). Glycans with the highest affinities for rCNL are listed. LDN motif is printed in boldface; human blood group A determinant trisaccharide is shaded black; tetrasaccharide A type 2 is shaded dark gray; A type 4 is shaded light gray; and A type 1 is underlined.

^a Mammalian printed array version 5.0 consisting of 611 glycan structures in replicates of six was used. A list of glycans and designation of spacer arms are available at the Consortium for Functional Glyconics website.

^b Relative fluorescence units (RFU) represent the degree of binding of fluorescently labeled streptavidin to biotin-labeled rCNL, which indicates the specificity of rCNL for individual glycans. The value represents the mean of four replicates (the highest and lowest points from six replicates have been removed).

^c At each concentration, a rank was determined for each glycan as 100 × (RFU of individual glycan/highest RFU value in the assay). Finally, average ranks were calculated for individual glycans, which are sorted in descending order.