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. 2011 Dec 22;287(13):10189–10199. doi: 10.1074/jbc.M111.303636

FIGURE 6.

FIGURE 6.

miR-29a inhibited PC-1 cell proliferation by targeting Nasp. A, CCK-8 assay of proliferation of PC-1 cells at 0, 48, or 96 h after transfection with pre-miR-NC, pre-miR-29a, or siRNA against Nasp, respectively. B, cell counting analysis of proliferation of PC-1 cells at 0, 24, 48, 72, or 96 h after transfection with pre-miR-NC, pre-miR-29a, or siRNA against Nasp, respectively. C, rescue experiments in PC-1 cells. PC-1 cells were co-transfected with pre-miR-29a and the tNASP expression vectors, together with wild-type (pcDNA3.1(+)-tNASP-3′UTR-WT) or mutant (pcDNA3.1(+)-tNASP-3′UTR-Mut1 and -2) 3′ UTR, respectively. The proliferation activities of PC-1 cells were measured with the CCK-8 kit 72 h after transfection. The result was represented as mean ± S.D. *, p < 0.05; ***, p < 0.001.