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InsP6 binding to PhyAsrH188C/C252S detected by changes in relative fluorescent intensity. The 5-IAF fluorophore was introduced at the periphery of the active site (H188C) near the Pc site. A, titration of PhyAsrH188C/C252S with InsP6. The binding curve suggests a maximal dissociation constant in the low micromolar range. Preliminary rapid kinetics measurements suggest a two-phase binding over 10 s (B) and 100 s (C).
