FIGURE 6.

MshB active site accessibility. The panels show the surfaces of the six MshB monomers from the overlaid structures in A for two different viewpoints. The surface colors correspond to locations of Tyr-142 shown in A, and the dark gray is the Tyr-142 side chain. The substrate GlcNAc-Ins is presumed to bind in the location of the two BOG molecules (colored in red and light gray) that are observed in the MshB-BOG structures (Fig. 1C). A, viewpoint 1 with the Tyr-142 side chain shown in dark gray. B, viewpoint 1, where the Tyr-142 side chain was truncated to Ala (dark gray). C, viewpoint 2 with the Tyr-142 side chain shown in dark gray. D, viewpoint 2, where the Tyr-142 side chain was truncated to Ala (dark gray).