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Effect of metal ions on pH dependence of wild-type MshB
| Me2+-MshBa,b | pKa1 | pKa2 | V/K at pH optimum |
|---|---|---|---|
| m−1s−1 | |||
| Zincc | 7.4 ± 0.07 | 10.5 ± 0.06 | 48 ± 1.5 |
| Cobalt | 7.2 ± 0.09 | 11.0 ± 0.09 | 56 ± 2.3 |
| Iron | 7.2 ± 0.09 | 10.5 ± 0.08 | 63 ± 2.3 |
| Nickel | 7.1 ± 0.03 | 11.1 ± 0.03 | 7.3 ± 0.1 |
| Manganese | 7.2 ± 0.12 | 11.0 ± 0.12 | 8.0 ± 0.4 |
a Apo-MshB was incubated with stoichiometric metal for 30 min prior to activity measurement.
b Substrate 5 mm GlcNAc.
c Data adapted from Ref. 14.
