FIGURE 1.
Complementary DNA sequence and structure of porcine Bim. A, deduced amino acid sequence for porcine BimEL aligned to its human and murine counterparts. Identical amino acids are indicated by asterisks, and amino acids with conserved similarities are indicated by dots or colons. The BH3 and dynein-binding domains are boxed. The COOH-terminal hydrophobic region is overlined. The amino acid numbers are indicated on the right. B, antibody that recognizes both murine and porcine BimEL proteins was used to conduct Western blotting on samples from murine and porcine ovary tissue. C, identification of multiple RT-PCR products of porcine Bim. The coding region of Bim cDNA was amplified by RT-PCR using total RNA from porcine lung, liver, and ovary and murine liver and ovary, using animal-specific primers. The PCR products were subjected to agarose gel electrophoresis and photographed. The segment size is marked on both sides. D, genomic organization of porcine Bim isoforms.