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. 2012 Feb 8;287(13):10684–10692. doi: 10.1074/jbc.M111.317792

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Rapid actin filament depolymerization by Spir. A, a co-sedimentation assay of 6 μm Spir-CD added to 6 μm F-actin. Spir-CD can be seen in the supernatant (S) (but not in the pellet (P)) with actin that has mostly depolymerized after a 15-min incubation. Representative EM image of actin incubated with a stoichiometric amount of Spir-CD for 15 min. Some short filaments remain, but the majority of the fields imaged have no F-actin. B, fluorescence curves of 5 μm F-actin (2.5% pyrene labeled actin) upon the addition of Spir-ABCD (concentrations as indicated) show rapid depolymerization. The actin polymerization was monitored for 1200 s before Spir was added. Depolymerization is dose-dependent and is complete above stoichiometric Spir concentrations. Filaments exhibit faster depolymerization in the presence of Spir than in the presence of sequestration agent, LatA.