TABLE 1.
Comparison of observed and theoretical sedimentation coefficients (in Svedbergs) of Spir constructs incubated with actin at stoichiometric ratios under polymerizing and nonpolymerizing conditions
The theoretical S values for actin oligomers were calculated as described under “Experimental Procedures” (+ salt indicates 2 mm MgCl2 and 50 mm KCl).
| Observed sedimentation coefficients | S | Theoretical sedimentation coefficients | S |
|---|---|---|---|
| Actin | 3.25 | Actin | 3.25 |
| Actin + Spir-CD | 3.80 | 2 actin prolated ellipsoid | 4.94 |
| Actin + Spir-CD (+ salt) | 4.65 | 3 actin prolated ellipsoid | 6.08 |
| Actin + Spir-ABCD | 5.80 | 3 actin oblate ellipsoid | 6.49 |
| Actin + Spir-ABCD (+salt) | 6.25 | 3 actin spherical | 6.76 |
| 4 actin prolated ellipsoid | 6.93 | ||
| 4 actin oblate ellipsoid | 7.86 | ||
| 4 actin spherical | 8.19 |