FIGURE 7.

The conversion of the type 1-related IFN-γ/STAT4 to the type 2-related IL-4/STAT6 is inducible in mCIITA-transgenic C57BL/6 mice. A, detection of the transgenic mCIITA gene in C57BL/6 mice by PCR as indicated as 500- and 800-bp bands is shown. Tg1 and Tg2 represent DNA samples from two individual transgenic mice. Wild-type littermate mice were set as controls (WT mice). B, a depressed percentage of the H-2 I-A/I-E⁺ splenocytes in the mCIITA mice compared with littermate non-transgenic mice is shown. I-A/I-E expression was determined by flow cytometry. An isotype-matched Ab was used as control. C, increased expression of IL-4 (type 2 cytokine) and repressed expression of IFN-γ (type 1 cytokine) in the mCIITA-transgenic C57BL/6 mice was detectable at the mRNA level by real-time PCR in splenocytes (upper row) and at the protein level by ELISA in the supernatants of cultured splenocytes (bottom row). D, increased STAT6 and decreased STAT4 were also determined by real-time PCR in the splenocytes of mCIITA mice.