FIGURE 3.
Dependence on active Gat1p for lipid particle accumulation in response to oleate. Cells lacking Gat1p (VZY31) were transformed with empty vector (pYES) or vector carrying wild type GAT1 or the catalytically dead mutant allele GAT1G261L. Transformants were grown to log phase in selective medium containing 2% galactose and then were used in serial dilution assays (A) or transferred to liquid medium containing 3.5 mm oleate (OA) for 24 h. Aliquots of oleate cultures were used for Western blot analysis using 5 μg of total protein from cell lysates (B) or cells were stained with Nile Red for fluorescence microscopy inspection (C). BF, bright field. Scale bar, 5 μm.