TABLE 1.
Metabolites whose levels were significantly changed by SeMet treatment
The mean values of the ratio (T30/T0) from three independent experiments are presented. The metabolic pathways were assigned according to the KEGG database. CoA, γ-glutamylcysteine, homocysteine, and urocanic acid were not detected in cell extracts after SeMet treatment. All compounds detected in CE-TOFMS analysis are listed in supplemental Table S2.
| Candidate compounds | -Fold | Related metabolic pathway |
|---|---|---|
| Homoserinelactone | 160 | |
| 1-Aminocyclopropane-1-carboxylic acid | 31 | Cysteine and methionine metabolism |
| Propanoate metabolism | ||
| GDP-mannose | 5.6 | Fructose and mannose metabolism |
| N-Glycan biosynthesis | ||
| Amino sugar and nucleotide sugar metabolism | ||
| Ribulose 5-phosphate | 3.6 | Pentose phosphate pathway |
| Pentose and glucuronate interconversions | ||
| Methane metabolism | ||
| Riboflavin metabolism | ||
| Vitamin B6 metabolism | ||
| Glycerophosphocholine | 3.1 | Glycerophospholipid metabolism |
| Ether lipid metabolism | ||
| Trehalose 6-phosphate | 3.1 | Starch and sucrose metabolism |
| Orotidine 5′-monophosphate | 0.21 | Pyrimidine metabolism |
| N-Acetylglucosamine 6-phosphate | 0.10 | Amino sugar and nucleotide sugar metabolism |
| Reduced glutathione | 0.06 | Cysteine and methionine metabolism |
| Glutathione metabolism | ||
| CoA | NDa | Fatty acid metabolism |
| Pantothenate and CoA biosynthesis | ||
| γ-Glutamylcysteine | ND | Glutathione metabolism |
| Homocysteine | ND | Cysteine and methionine metabolism |
| Sulfur metabolism | ||
| Urocanic acid | ND | Histidine metabolism |
a ND, not detected in T30 samples.