FIGURE 1.

Two-dimensional ¹H-¹⁵N HMQC spectra of ¹⁵N-labeled hNaa50p in absence or presence of acetyl-CoA or CoA. A, two expanded regions of the two-dimensional ¹H-¹⁵N HMQC spectrum of ¹⁵N-labeled free hNaa50p. B, overlay of the HMQC spectra of hNaa50p (black) and hNaa50p with acetyl-CoA (blue). C, overlay of the HMQC spectra of hNaa50p (black) and hNaa50p with CoA (green). For clarity, the complete peak identity is shown only in A, whereas peaks with significant chemical shift changes upon acetyl-CoA or CoA binding are shown in B and C. The side chain signals of Asn and Gln present in the expansions in the left panels (severely overlapped peaks) are not assigned. Negative signals (red) are either noise or side chain signals of Arg. There was no effect observed on these peaks. Peaks with unknown identity are indicated with red asterisks. Note that the extraction in the right panel in B includes one of the autoacetylated lysine side chains, the peak marked K-N^ζH^ζ (not assigned).