FIGURE 5.

miR-217 promotes ethanol-mediated inhibition of SIRT1 protein and activity in AML-12 hepatocytes. A, cell extracts of AML-12 cells transfected with plasmids of control vector, miR-217, or Sc-miR-217 with or without ethanol (50 mm) were immunoblotted with a SIRT1, p-AMPKα, AMPKα, or β-actin antibody. B, relative protein levels of SIRT1. C, relative levels of p-AMPKα/AMPKα ratio. D, SIRT1 deacetylase activity was measured in AML-12 cells transfected with plasmids of control vector, miR-217, or Sc-miR-217 with or without ethanol (50 mm). SIRT1 deacetylase activity is represented as arbitrary fluorescence units (AFU). All data are means ± S.E. (error bars) from at least 3–5 experiments. Means without a common letter differ; p < 0.05.