Table. Primer sets for polymerase chain reaction amplification of Pneumocystis gene loci commonly used for molecular typinga.
| Gene locus | Primer sequence | Reference | |
|---|---|---|---|
| β-tubulin | |||
| Forward | 5´ TCA TTA GGT GGT GGA ACG GG 3´ | (31) | |
| Reverse | 5´ ATC ACC ATA TCC TGG ATC CG 3´ | (31) | |
| DHPS (nested) | |||
| 1st round | DHPS F1 | 5´ CCT GGT ATT AAA CCA GTT TTG CC 3´ | (28) |
| DHPS B45 | 5´ CAA TTT AAT AAA TTT CTT TCC AAA TAG CAT C 3´ | (29) | |
| 2nd round | DHPS AHUM | 5´ GCG CCT ACA CAT ATT ATG GCC ATT TTA AAT C 3´ | (29) |
| DHPS BN | 5´ GGA ACT TTC AAC TTG GCA ACC AC 3´ | (29) | |
| ITS (nested) | |||
| 1st round | 1724F | 5´ AAG TTG ATC AAA TTT GGT C 3´ | (22) |
| ITS2R | 5´ CTC GGA CGA GGA TCC TCG CC 3´ | (22) | |
| 2nd round | ITS1F | 5´ CGT AGG TGA ACC TGC GGA AAG GAT C 3´ | (22) |
| ITS2R1 | 5´ GTT CAG CGG GTG ATC CTG CCT G 3´ | (22) | |
| ITS (nested) | |||
| 1st round | N18SF | 5´GGT CTT CGG ACT GGC AGC 3´ | (26) |
| N26SRX | 5´ TTA CTA AGG GAA TCC TTG TTA 3´ | (26) | |
| 2nd round | ITSF3 | 5´ CTG CGG AAG GAT CAT TAG AAA 3´ | (24) |
| ITS2R3 | 5´> GAT TTG AGA TTA AAA TTC TTG 3´ | (24) | |
| MSG | |||
| Forward | GK242 | 5´ TAT TTC TTG TAT CTA TGC GCT 3´ | (33) |
| Reverse | GK244 | 5´ TCC GCG CAA AAA TAA GCA CT 3´ | (33) |
| mt LSU rRNA (nested) | |||
| 1st Round | pAZ102-H | 5´ GTG TAC GTT GCA AAG TAC TC 3´ | (28) |
| pAZ102-E | 5´ GAT GGC TGT TTC CAA GCC CA 3´ | (28) | |
| 2nd Round | pAZ102-X | 5´ GTG AAA TAC AAA TCG GAC TAG G 3´ | (25) |
| pAZ102-Y | 5´ TCA CTT AAT ATT AAT TGG GGA GC 3´ | (25) | |
| Nuclear 26S rRNA | |||
| Forward | 5´ GAA GAA ATT CAA CCA AGC 3´ | (31) | |
| Reverse | 5´ ATT TGG CTA CCT TAA GAG 3´ | (31) | |
aDHPS, dihydropteroate synthase; ITS, internal transcribed spacer; MSG, major surface glycoprotein; mt LSU, mitochondrial large subunit.