Table 5. Characteristics of 22 isolates of atypical enteropathogenic Escherichia coli identified during this study.
Strain | Sou-rcea | Serotype | Intimin type | Result of PCRb |
|||||||
---|---|---|---|---|---|---|---|---|---|---|---|
bfpA | aggR | efa1 | astA | lpfD | Adhesion patterna | FAS assay | HEp-2 cell invasionc | ||||
W7 | N | O161:H40 | β | – | – | – | – | – | NA | – | 0.01 |
W85-2 | N | OR:H- | γ | – | – | – | – | – | AA | + | 0.02 |
W114 | N | O107:H8 | ι | – | – | – | – | – | IA | + | 0.02 |
W143 | N | Ont:H5 | ε | – | – | – | – | – | IA | + | < 0.01 |
W145 | N | O55:H6 | γ | – | – | + | + | – | AA | + | 0.03 |
W154 | N | O139:H14 | β | – | – | – | – | – | AA | + | < 0.01 |
W185 | N | Ont:H21 | θ | – | – | – | – | – | IA | + | 0.02 |
W208 | N | Ont:H4 | β | – | – | – | – | – | IA | + | 0.08 |
W761 | N | O124:H40 | λ | – | – | – | – | – | NA | – | 0.07 |
W902 | N | O125:H6 | α2 | – | – | – | – | – | IA | + | 0.06 |
W914 | N | O125:H6 | α | – | – | – | – | – | AA | + | 0.07 |
W1040 | G | O15:Hnt | β | – | – | + | – | + | LAL | + | 0.10 |
W1056 | G | O55:H7 | γ | – | – | + | + | – | IA | + | 0.77 |
W1068 | G | O51:H49 | α | – | – | – | – | – | AA | + | < 0.01 |
W1082 | G | Ont:H6 | β | – | – | – | – | – | AA | + | 0.01 |
W1092 | G | OR:H- | η/ε | – | – | – | – | + | IA | + | < 0.01 |
W1108 | G | O172:H4 | θ | – | – | – | – | – | IA | + | 0.03 |
W1118 | G | O126:H6 | α | – | – | – | – | – | IA | + | 0.01 |
W1120 | G | Ont:H34 | α | – | – | – | – | – | IA | + | 0.01 |
W1134 | G | Ont:H6 | θ | – | – | – | – | – | NA | – | 0.01 |
W1585 | G | Ont:H40 | θ | – | – | – | – | – | NA | – | 0.02 |
W1706 | G | Ont:H6 | α | – | – | – | – | – | AA | + | 0.01 |
E128012 | C | O114:H2 | β | – | – | + | – | + | IA | + | 0.32 |
aN, no symptoms; G, gastroenteritis; C, control strain; Ont, O nontypable (O1–O181); Hnt, H nontypable (H1–H56); NA, nonadherent; AA, aggregative adherence pattern; IA, indeterminate adherence pattern; LAL, localized-like adherence pattern; FAS, fluorescent actin staining. bPCR, polymerase chain reaction; +, positive; –, negative. cData are the number of bacteria recovered from HEp-2 cells after treatment with gentamicin as a percentage of the total number of cell-associated bacteria and are the mean of two separate assays.