Table 1. Use of animals.

Study	Time after injury	Number of injured animals per group
Cyclin A, cyclin D1, and fodrin expression. Figures 1A to 1C	6 hours	N=3
Fine-motor coordination (beam walk) and cognitive (Morris water maze) assessment. Figures 2A and 2B	21 days	N=11–12
Lesion volume (unbiased stereology—cresyl-violet staining). Figure 2A to 2C	21 days	N=4–5
Neuronal degeneration (Fluoro-Jade B staining). Figure 4A	24 hours	N=3
Neuronal loss quantification in the cortex and hippocampus (unbiased stereology—cresyl-violet staining). Figure 4C, 4D to 4F	24 hours 7 days 21 days	N=3–4 N=3–4 N=3–4
Microglial activation quantification and classification in the cortex (unbiased stereology—Iba-1 staining). Figure 5A to 5D	24 hours 7 days 21 days	N=3–4 N=3–4 N=3–4
Fine-motor coordination (beam walk). Figure 6A	21 days	N=6
Microglial activation quantification and classification in the cortex (unbiased stereology—Iba-1 staining). Figure 6B and 6C	24 hours 7 days 21 days	N=3–4 N=3–4 N=3–4