Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2004 Jul;10(7):1300–1303. doi: 10.3201/eid1007.030678

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.

PMC Copyright notice

LightCycler amplification curves of the three single SARS-coronavirus (CoV)-specific assays and the combined assay. Five hundred genome equivalents (GE) of SARS-CoV were amplified with the three single assays-NS pp1a, NS pp1ab, and SS GP-as well as with the combination of the three assays. No fluorescence signal was observed for control pathogens. In the inset, the respective agarose gel analysis of the amplification products in a 3% gel is shown. Lane 1: 50-bp marker, lane 2: 500 GE SARS-CoV amplified with the assay combination (products of assays NS pp1ab and SS GP are not clearly distinguishable because of sizes of 88 bp and 79 bp, respectively), lane 3: human CoV 229E, lane 4: respiratory syncytial virus, lane 5: rhinovirus, lane 6: human metapneumovirus, lane 7: Mycoplasma pneumoniae, or lane 8: Chlamydia pneumoniae.