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Primers used for RT-PCR
| Target gene | primer sequence |
|---|---|
| TNF-α | F: 5'-AGCCCACGTCGTAGCAAACCACCAA-3' |
| R: 5'-AACACCCATTCCCTTCACAGAGCAAT-3' | |
| IL-6 | F: 5'-CATGTTCTCTGGGAAATCGTGG-3' |
| R: 5'-AACGCACTAGGTTTGCCGAGTA-3' | |
| COX-2 | F: 5'-TTTGATTAGTACTGTAGGGTTAATG-3' |
| R: 5'-TTTGATTAGTACTGTAGGGTTAATG-3' | |
| iNOS | F: 5'-CCTCCTCCACCCTACCAAGT-3' |
| R: 5'-CACCCAAAGTGCTTCAGTCA-3' | |
| β-actin | F: 5'-TGGAATCCTGTGGCATCCATGAAA-3' |
| R: 5'-TAAAACGCAGCTCAGTAACAGTCCG-3' |
F, forward; R, reverse
