Table 4.

Primers of the 20 SSR loci used in the analysis

Locus name	EMBL Accession No.	Primer sequence Forward	Primer sequence Reverse	Repeat motifs	Ta (°C)
mCrCIR02A09	FR677568	ACAGAAGGTAGTATTTTAGGG	TTGTTTGGATGGGAAG	(GA)9	55
mCrCIR03C08	FR677576	CAGAGACAGCCAAGAGA	GCTTCTTACATTCCTCAAA	(GA)16	55
mCrCIR02D04B	FR677564	CTCTCTTTCCCCATTAGA	AGCAAACCCCACAAC	(GT)10(GA)7	50
mCrCIR02D09	FR677569	AATGATGAGGGTAAAGATG	ACCCATCACAAAACAGA	(GA)10	55
mCrCIR03D12a	FR677577	GCCATAAGCCCTTTCT	CCCACAACCATCACC	(GT)10(GA)6	50
mCrCIR03G05	FR677578	CCACACAGGCAGACA	CCTTGGAGGAGCTTTAC	(GAAA)3(GA)11	50
mCrCIR02G12	FR677575	AAACCGAAATACAAGAGTG	TCCACAAACAATACAACG	(GA)14	55
MEST121	DY275927	TCCCTATCATCGGCAACTTC	CAATAATGTTAGGCTGGATGGA	(TAA)9	55
MEST431	DY285140	GAGCTCAAAACAATAGCCGC	CATACCTCCCCGTCCATCTA	(CAG)7	55
MEST458	DY283417	CCCCCTCTTTTTCTCTTCCA	TTCTGGGCTGGTAGGTTCAG	(TC)12	55
mCrCIR01C06	AJ567393	GGACCACAACAAAGACAG	TGGAGACACAAAGAAGAA	(GA)9	50
mCrCIR01C07	AJ567394	GTCACTCACTCTCGCTCTTG	TTGCTAGCTGCTTTAACTTT	(CT)10	55
mCrCIR01D06a	AM489734	GATCAAAACATTATTCCAA	TTTTTCATCAACAAGACTG	(CA)12	50
mCrCIR01E02	AM489735	TGAATGGTACGGGAAATGC	CAGGGTCGGTGGAGAGGAT	(GA)16	55
mCrCIR01F04a	AM489736	AAGCATTTAGGGAGGGTCACT	TGCTGCTGCTGTTGTTGTTCT	(CT)13CC(CT)7	55
mCrCIR01F08a	AM489737	ATGAGCTAAAGAGAAGAGG	GGACTCAACACAACACAA	(GAAT)6	50
mCrCIR01H05	AJ567401	AAAACAACCAAAAGGACAAGATT	TTCAAACTAAACAAACCAACTCG	(GA)9	55
mCrCIR06B05	AM489744	GAACGATGGAATGAAGTG	ATGTTGATTACGAGACCTT	(GA)26	55
mCrCIR07D06	FR677581	CCTTTTCACAGTTTGCTAT	TCAATTCCTCTAGTGTGTGT	(TAAT)4N(TG)8(GA)11	55
mCrCIR07D07	AM489748	GCTGATGATACGCACGAACC	CACAACGCCAAAAACGACTC	(GA)10	55

Ta: annealing temperature. MEST: Marker selected from Clementine Expressed Sequence Tag, mCrCIR: Microsatellite selected from genomic sequences of C. reticulata at CIRAD