Figure 1.

ORO staining of cytoplasmic neutral lipids. Freshly isolated PBMCs were stained with ORO to demonstrate neutral lipids and counterstained with haematoxylin for nuclei. Cells were then examined by light microscopy and two different fields per sample were imaged. Red ORO intensity was measured in these two fields using NIH Image J software. Panel (a) shows representative images of ORO stained (on the left) and their corresponding ROI (on the right). Panel (b) shows quantization of red intensity expressed as the percentage of pixel intensity ± SD⁄ total area of each image. Analysis of variance (ANOVA) shows significant statistically differences among the four groups (P = 0.000).