Figure 1.

Features of two fluorescent dyes. (A) Additive fluorescent emissions of SYBR Green II. For each concentration level, three samples were prepared individually in triplets: 50% DNA (50% purified DNA + 50% pure water), 50% cDNA (50% purified cDNA + 50% pure water), and 50% cDNA + 50% DNA. Each was mixed in equal quantity with the dye solution (10 μl SYBR Green II buffer +10 μl sample) at 25°Cfor 5 min before taking measurements. Standard errors are indicated by black bars. (B) Fluorescent emissions of Picogreen in mixed solutions. Pure cDNA (100% cDNA), DNA (100% DNA), and their mixtures in different proportions were prepared in triplets, and mixed with Picogreen solution (10 μl Picogreen buffer +10 μl sample) before measurements were taken. Bar indicates the standard error of each mean.