Table 2.
Quantitation of absolute numbers of bh2c transcript copies in the corolla samples of Ipomoea purpurea
| Sample name | Type | Ct | Given concentration (copies/μl) |
Calculated Concentration (copies/μl) |
cDNAs concentration* (pg/μl) |
Transcript copies/fg cDNAs** |
|---|---|---|---|---|---|---|
| bh2c-1 | Standard | 6.67 | 117363.39 | 128533.38 | ||
| bh2c-2 | Standard | 10.21 | 11736.33 | 10758.19 | ||
| bh2c-3 | Standard | 13.24 | 1173.63 | 1284.77 | ||
| bh2c-4 | Standard | 16.83 | 117.36 | 104.04 | ||
| bh2c-5 | Standard | 19.93 | 11.74 | 11.80 | ||
| bh2c-6 | Standard | 23.4 | 1.17 | 1.04 | ||
| bh2c-7 | Standard | 26.31 | 0.117 | 0.135 | ||
| bh2c 2008-9-23 | Unknown | 13.22 ± 0.03 | 1302.40 ± 24.43 | 9101.52 | 143.10 ± 2.68 | |
| bh2c 2008-9-24 | Unknown | 20.02 ± 0.04 | 11.14 ± 0.30 | 10285.83 | 1.08 ± 0.03 | |
| bh2c 2008-9-25 | Unknown | 14.08 ± 0.02 | 714.67 ± 8.61 | 10582.80 | 67.53 ± 0.81 | |
| bh2c 2008-9-26 | Unknown | 13.95 ± 0.01 | 784.27 ± 6.37 | 6614.55 | 118.57 ± 0.96 | |
| bh2c 2008-9-27 | Unknown | 13.27 ± 0.01 | 1261.69 ± 5.28 | 10534.68 | 119.77 ± 0.50 |
*The concentrations of cDNAs (pg/μl) were derived from Table 1 after taking 50 × factor into account.
** The copy number was estimated via equation (1). Standard errors (± se) are based on triplet measurements.