Table 3.
CBA estimates of expression variations of reference and target genes in developing Ipomoea purpurea petals across environments
| Variation source | Genotype | Sample size | Gene | Mean expression (transcript copies/pg cDNA) | Coefficient of Variation |
|---|---|---|---|---|---|
| From 60 HBF* until flowering | III6D | 31 | IpACTIN4 | 429.8 | 159% |
| IpGAPDH2 | 247.0 | 106% | |||
| IpDFRB-fl1 | 1710.5 | 114% | |||
| From 90 HBF until flowering | GZKL | 30 | IpACTIN4 | 133.6 | 80% |
| IpGAPDH2 | 327.1 | 72% | |||
| IpF3'H-blue | 136.1 | 84% | |||
| IpWD1-a | 8.8 | 67% | |||
| 36 HBF among four days | II8II2 | 4 | IpACTIN4 | 86.0 | 66% |
| IpGAPDH2 | 45.2a | 27% | |||
| IpF3'H-blue | 20.1 | 80% | |||
| IpMYB1-a | 19.4 | 70% | |||
| II8SX | 4 | IpACTIN4 | 53.8 | 52% | |
| IpGAPDH2 | 4.0b | 113% | |||
| IpCHI-fl1 | 44.9 | 93% | |||
| S2Y6 | 4 | IpACTIN4 | 39.2 | 95% | |
| IpGAPDH2 | 8.1b | 139% | |||
| IpANS-f | 11.8 | 102% | |||
| YNSX | 4 | IpACTIN4 | 21.7 | 126% | |
| IpGAPDH2 | 6.2b | 165% | |||
| IpF3H-1 | 4.8 | 178% | |||
| Ip3GT-b | 127.0 | 92% | |||
| Same day at four stages | SXSX2-2 | 4 | IpACTIN4 | 128.6 | 119% |
| IpGAPDH2 | 258.8 | 124% | |||
| IpCHSD-us1 | 585.5 | 137% | |||
| SXSX2-8 | 4 | IpACTIN4 | 105.1 | 41% | |
| IpGAPDH2 | 238.5 | 40% | |||
| IpCHSD-mex9 | 474.5 | 65% | |||
| IpWD1-b | 4.2 | 51% |
*HBF refers to hours before flowering. For genotypeII8II2, four days were 6, 11, 16 and 21 of September 2010. For genotypeSXSX2-2 &-8, four stages were at 9 am, 10 am, 11 am, and 12 pm of 28 September 2010.
a,b Significant Wilcoxon two-sample test was indicated between superscripts, P = 0.014.