Table 1. dhs-16 epistasis and synergy experiments.
Genotype | Daf-c at 25°C ± SE(%)a | N b | Daf-c at 27°C ± SE(%)a | N b | Mig at 20°C ± SE(%) NG-cholc | N b |
WT | 0±0 | 1,792(3) | 13±3 | 1,869(3) | 0±0 | 146(3) |
daf-36(k114) | 0±0 | 467(2) | 91±5 | 2,849(3) | 59±11 | 136(3) |
dhs-16(tm1890) | 0±0 | 492(3) | 65±11 | 1,252(3) | 37±13 | 158(3) |
dhEx396(dhs-16::gfp) | nd | 0±0 | 174(3) | 0±0 | 122(3) | |
dhs-16; dhEx396 | nd | 1±1 | 335(3) | 0±0 | 150(3) | |
daf-5(e1386) | nd | 0±0 | 1,136(3) | nd | ||
dhs-16; daf-5 | nd | 59±3 | 706(3) | nd | ||
daf-16(mu86) | nd | 0±0 | 1,849(3) | nd | ||
dhs-16; daf-16 | nd | 42±3 | 2,390(3) | nd | ||
daf-12(rh61rh411) | nd | 0±0 | 1,223(3) | nd | ||
dhs-16; daf-12 | nd | 0±0 | 1,072(3) | nd | ||
daf-9(k182) | 0±0d | 1,222(2) | 66±29 | 909(2) | 18±0e | 68(1) |
daf-36;daf-9 | 100±0d | 356(2) | nd | nd | ||
dhs-16; daf-9 | 14±9d | 947(2) | nd | 64±0e | 66(1) | |
dhs-16; daf-36 | 33±4 | 968(2) | 77±7 | 224(2) | 78±2e | 50(2) |
daf-2(e1370) | 0±0d | 370(2) | nd | nd | ||
dhs-16;daf-2 | 3±3d | 150(2) | nd | nd | ||
hsd-1(mg433) | 0±0 | 964(2) | 0±0 | 909(2) | nd | |
dhs-16; hsd-1 | 68±21 | 628(2) | 95±3 | 565(2) | nd | |
ncr-1(nr2022) | 0±0 | 660(2) | 47±16 | 342(2) | 0±0 | 78(2) |
dhs-16; ncr-1 | 0±0 | 550(2) | 60±3 | 295(2) | 41±7e | 50(2) |
nd, not determined.
Dauers formed under reproductive growth conditions.
Number of experiments is given in parentheses.
Hermaphrodite distal tip cells that fail to turn in L3, n>50 cells, grown on NGM without added cholesterol.
Measured at 20°C.
Percentage Mig on normal NGM plates with cholesterol.