Figure 6. Mutation of buried Leu residues in the G-helix shortens the half-life of apoMb.

(A) Apo L115C and (B) apo L104C mutant proteins were incubated with proteasome in the presence or absence of MG132. Rate of degradation was followed by SDS-PAGE and quantified as described in methods. Data represent mean values of at least three independent experiments ±S.D.