Skip to main content
. 2012 Apr 10;7(4):e34957. doi: 10.1371/journal.pone.0034957

Figure 3. DIM prevents cell cycle progression in VSMCs.

Figure 3

VSMCs were grown with DIM (25 µM) in the absence or presence of PDGF-BB (20 ng/ml) for 24 h, and cell cycle distribution was evaluated with flow cytometric analysis. A. Representative cell cycle profiles are shown. B. Quantification of VSMCs in the G0/G1, S, and G2/M phases, as determined by flow cytometric evaluation, is shown (#P<0.01 versus control group; *P<0.01 versus PDGF alone; n = 3). C. Cell cycle protein expression was measured with western blot analysis. GAPDH detection served as a loading control.