Table 1. PCR Primers for evaluating splice variation.
Exons Amplified | Product Size | Primer | Primer Sequence |
1a-5(For CLU1) | 596 bp | Exon 1a Forward | GCGAGCAGAGCGCTATAAAT |
Exon 5 Reverse | GATGCGGTCACCATTCATC | ||
1b-5(For CLU2) | 567 bp | Exon 1b Forward | AGATGGATTCGGTGTGAAGG |
Exon 5 Reverse | GATGCGGTCACCATTCATC | ||
4–6 | 598 bp | Exon 4 Forward | AGAGTGTAAGCCCTGCCTGA |
Exon 6 Reverse | AGACAAGATCTCCCGGCACT | ||
5–9 | 675 bp | Exon 5 Forward | GGACATCCACTTCCATAGCC |
Exon 9 Reverse | ACTTGGTGACGTGCAGAGC |