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. 2012 Apr 10;7(4):e34478. doi: 10.1371/journal.pone.0034478

Figure 3. Ndfip1 CD4-cKO mice treated with antibiotics for 2 weeks do not show decreased eosinophilia or reduced inflammation in the esophagus.

Figure 3

(A) The average 16s rDNA copies/nanogram of total stool DNA was quantified by qPCR at day 0 and day 13 of treatment with sucralose alone (n=5) or sucralose and antibiotics (n=4). Error bars illustrate SD. (B) Weights of mice treated with sucralose alone (−ABX=solid line) or antibiotics and sucralose (+ABX=dashed line) over the course of the 13 day treatment. (C) Representative H & E stained histological sections of the esophagus and small bowel of Ndfip1-cKO mice after 13 days of treatment with sucralose alone or antibiotics and sucralose are shown. (D) Percentages of activated CD4+ T cells in the spleens of mice treated with sucralose only (untreated) or with antibiotics and sucralose (treated) as determined by flow cytometric analysis. Each dot represents a single mouse.