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. 2012 Apr 10;7(4):e35005. doi: 10.1371/journal.pone.0035005

Figure 1. APECED patients with skin features have increased anti-melanoma antibody levels.

Figure 1

Cell lines were stained with serum obtained from APECED patients (2.D.G, 3.D.G., 4.U.G., 5.U.C., 6.M.R., 8.S.S., 12.F.M., 14.U.T.) diluted 1/500. Mm9, mm25, sk.mel23, sk.mel28, A2058 are human melanoma lines, TE671 a human rhabdomyosarcoma, and B16F10 murine melanoma cell line. Background using normal human serum was substracted and mean fluorescence indicated as follows −<0.5, +0.5–0.75, ++0.75–1, +++>1. The secondary antibodies used were A anti-human IgM FITC and B anti-human IgG FITC.