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. 2011 Jul 1;2(3):125–141. doi: 10.4161/self.17882

Figure 4.

Figure 4

PGRP-LC expression is not affected by the presence of high concentration of dead bacteria or protease-deficient E. coli. The expression of FLAG-tagged PGRP-LCa/x in S2 cells in response to dead E. coli/Salmonella/Staph and protease-deficient BL21(DE3) infection was determined by western blot analysis. (A), (B), (C), (D) and (E) None of the structurally intact but dead bacteria (either paraformaldehyde-fixed or ethanol-fixed E. coli, Salmonella or Staph) triggered any PGRP-LC cleavage at very high infection ratio of 50:1 (10-fold higher concentration than live bacterial infection). (F) A protease-deficient E. coli strain, BL21(DE3), was used to infect S2 cells stably expressing FLAG-tagged PGRP-LCa/x proteins. No significant receptor cleavage was detected at a high infection ratio of 60:1. Anti-FLAG-M5 mAb was used to detect PGRP-LCa/x expression and Actin was used as a loading control.