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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Breast Cancer Res Treat. 2011 Dec 9;133(3):1037–1048. doi: 10.1007/s10549-011-1902-7

Figure 3.

Figure 3

Effect of ethanol on tumor angiogenesis. Mice received implantation of E0771 cells and ethanol exposure as described above. Twenty four days following ethanol exposure, the mice were sacrificed and mammary tumors were removed. A: Mammary tumor tissues were fixed and sectioned. The sections were processed for CD31 immunohistochemistry (IHC) for the detection of microvessels as described under the Materials and Methods. Bar = 50 μm. B: Microvessels in tumor tissues were detected by CD31 IHC and the average microvessel density (AMVD) was determined by counting the number of microvessels per mm2 area. At least 6 areas from each group were examined. * denotes a significant difference from the control.