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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Mod Pathol. 2012 Jan 6;25(5):758–766. doi: 10.1038/modpathol.2011.191

Figure 4.

Figure 4

AKR1B10 silencing resulted in induction of apoptosis and inhibition of cell proliferation. (A) Western blot analysis showed that increased level of cleaved caspase-8 protein, a marker of apoptosis, in Panc10.05 cells with AKR1B10 silencing. (B) Immunocytochemical analysis of cell apoptosis using activated caspase-3 in the scambled siRNA control (B1) and those with siRNA silencing of AKR1B10 expression (B2). Silencing of AKR1B10 expression resulted in increased activated caspase-3 labeled cell apoptosis as demonstrated by the arrows in the bottom image as well as the small size of the cell clones. The number of cells per clone was further counted and revealed that a significant decrease in the number of cells per clone were identified in Panc10.05 carcinoma cells with AKR1B10 silencing when compared to the parental control (18±9 vs. 32±11, p=0.048).