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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Mod Pathol. 2012 Jan 6;25(5):758–766. doi: 10.1038/modpathol.2011.191

Figure 5.

Figure 5

Western blot analysis of prenylated proteins including HDJ2 and KRAS as well its downstream signals. Increased non-farnesylated HDJ2 protein (upper black arrow) and decreased membrane-bound KRAS protein were observed in the siRNA AKR1B10 pancreatic cancer cell line when compared to the scrambled siRNA control. Increased levels of membrane-bound E-cadherin (lower black arrow) and decreased levels of KRAS downstream effectors, phosphor-ERK and phosphor-MEK1/2, were also found. Flottin-2 was used as a loading control for membrane-bound proteins, while β-actin is used for cytosolic proteins.