Figure 1. Circulating levels of VWF and FVIII in the infused animals.

Animals were infused with human VWF and /or FVIII from various sources at 50 IU/kg using varying routes. Plasma samples were collected at various time points for VWF:Ag ELISA or FVIII:C assay. Fig. 1A shows the levels of VWF in FVIII^null mice over a 24 hour period after infusion of Alphanate containing plasma derived human VWF (phVWF) and FVIII (phFVIII). A human-specific VWF:Ag ELISA assay was used. Fig. 1B shows the first 4 hours after infusion of Alphanate in another experiment. Fig. 1C shows the levels of VWF in VWF^null mouse plasma after infusion of recombinant human VWF (rhVWF). Fig. 1D shows the levels of FVIII in FVIII^null animals that were infused with Alphanate. Fig. 1E shows the levels of FVIII in FVIII^null mice that were infused with recombinant human FVIII (rhFVIII). Fig. 1 F. shows that endogenous murine FVIII was restored in VWF^null mice when rhVWF was infused whether by IP or IV injection.