Fig. 2.
HPLC chromatogram of α-KGA-derived products in synaptic vesicles. Synaptic vesicle fraction SV-A′ was incubated with 30 μM [14C]α-KGA for 15 min in the presence of 2 mM L-aspartate and 2 mM ATP, and filtered. Radioactive compounds on the filter were analyzed by HPLC, as described in Materials and Methods. Authentic L-[3H]glutamate was used to identify the major radioactive peak as glutamate. Glu, glutamate.