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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Stem Cells. 2012 May;30(5):956–964. doi: 10.1002/stem.1064

Figure 2.

Figure 2

miR-125b protects hMSCs against anoikis. (A) miR-125b expression levels in adherent (Att) or suspended (Sus) hMSCs transfected with miR-125b antagonist (si-miR) (B) Knockdown of miR-125b decreased viability of hMSCs held in suspension. Viability assayed by trypan blue exclusion and reported as percent of cells versus total number seeded (C) Imaging hMSC survival by live (Green cytosolic) versus dead (punctate red nuclear) staining. Note that red fluorescence of siGLO causes some viable cells to be double labeled (yellow). These are easily distinguished from dead cells that exhibit punctate nuclear staining. (D) Knockdown of miR-125b had no effect on cell survival under adherent culture conditions. Percent of seeded exceeds 100% due to cell proliferation. (E) Knockdown of miR-125b promoted apoptosis (caspase 3 activity) in suspended hMSCs. * denotes p<0.05 for Student’s t-test.