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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Stem Cells. 2012 May;30(5):956–964. doi: 10.1002/stem.1064

Figure 5.

Figure 5

Upregulation of miR-125b in response to suspension is a stem-cell specific phenomenon. (A) miR-125b was expressed at low levels in HUVECs and could not be induced by cell suspension. Dotted line shows basal level of miR-125b expression in attached hMSCs (B) Exogenous miR-125b improved HUVEC survival in suspension conditions (75.2% viability compared to 51.9% of control cells following 3 hrs of suspension and replating) (C) Exogenous miR-125b decreased caspase 3-activity in suspended HUVECs (D) HUVECs did not sustain ERK activation in response to cell suspension; however, exogenous miR-125b elevated phospho-ERK levels (E) miR-125b expression was upregulated upon reprogramming of MEFs to an iPS-like state, but not by cell suspension (F) Qualitative model of how miR-125b regulates stem cell survival in low adhesion contexts. * denotes p<0.05 for Student’s t-test